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Coriell Institute for Medical Research
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European Collection of Authenticated Cell Cultures
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National Centre for Cell Science
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Image Search Results
Journal: Molecular cell
Article Title: Transcription Factor-Directed Re-Wiring of Chromatin Architecture for Somatic Cell Nuclear Reprogramming Toward Trans-differentiation
doi: 10.1016/j.molcel.2019.07.036
Figure Lengend Snippet: A, Experimental design. IMR90 fibroblasts containing doxycycline-inducible MYOD or EMPTY vector were treated with doxycycline (dox) for 24hrs in growth media (GM) prior differentiation stimuli (DM) with doxycycline for 72hrs. Experiments were always performed at these time points, unless otherwise stated.
Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS Human female lung primary fibroblasts isolated at 16 weeks gestation:
Techniques: Plasmid Preparation
Journal: Molecular cell
Article Title: Transcription Factor-Directed Re-Wiring of Chromatin Architecture for Somatic Cell Nuclear Reprogramming Toward Trans-differentiation
doi: 10.1016/j.molcel.2019.07.036
Figure Lengend Snippet: A, Scheme of the experimental approach used for all experiments in Fig. 6, EMPTY or MYOD IMR90 were transfected with plasmid encoding dCAS9 and specific gRNAs 36hours, then cells were treated with doxycycline for 24h in GM.
Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS Human female lung primary fibroblasts isolated at 16 weeks gestation:
Techniques: Transfection, Plasmid Preparation
Journal: Molecular cell
Article Title: Transcription Factor-Directed Re-Wiring of Chromatin Architecture for Somatic Cell Nuclear Reprogramming Toward Trans-differentiation
doi: 10.1016/j.molcel.2019.07.036
Figure Lengend Snippet: A, Time course analysis of Myod1 expression in doxycycline-treated IMR90 cells. Representative immunofluorescence images of IMR90 cells stained for MYOD. The nuclei were stained with DAPI.
Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS Human female lung primary fibroblasts isolated at 16 weeks gestation:
Techniques: Expressing, Immunofluorescence, Staining
Journal: Molecular cell
Article Title: Transcription Factor-Directed Re-Wiring of Chromatin Architecture for Somatic Cell Nuclear Reprogramming Toward Trans-differentiation
doi: 10.1016/j.molcel.2019.07.036
Figure Lengend Snippet: A, Scheme of the experimental approach used for all experiment in Fig. 5, EMPTY or MYOD IMR90 were exposed to doxycycline for 24h in GM followed by additional 48h of with/out doxycycline (ON/ON, ON/OFF).
Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS Human female lung primary fibroblasts isolated at 16 weeks gestation:
Techniques:
Journal: Molecular cell
Article Title: Transcription Factor-Directed Re-Wiring of Chromatin Architecture for Somatic Cell Nuclear Reprogramming Toward Trans-differentiation
doi: 10.1016/j.molcel.2019.07.036
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS Human female lung primary fibroblasts isolated at 16 weeks gestation:
Techniques: Recombinant, Transfection, Bicinchoninic Acid Protein Assay, Plasmid Preparation, Software, Microscopy